Immortalized clonal human myoblast line "M007"

Immortalized human myoblast cell line obtained from a healthy individual
Technology No. 2021-256

Applications

  • Research and testing of myogenesis
  • Myotube development
  • Differentiation
  • Drug development

Technology Overview

Immortalized human myoblast cell line from primary myoblasts obtained from the muscle biopsy of a healthy individual.The cells were immortalized by overexpressing hTERT, CCND1 and a mutant CDK4, using two vectors pLV-hTERT-IRES-hygro and pbabe-cyclinD1+CDK4R24C from Addgene. These cells can grow indefinitely and can be differentiated into myotubes in vitro, by changing the medium to "differentiation medium", essentially withdrawing growth factors

Technical Information


Organism: Homo sapiens (human)
Tissue: Skeletal Muscle
Disease: Normal
Karyotype: 46XX [20/20 normal metaphase spreads]
Product format:Frozen

Desired Partnerships

This cell line is fully developed and available for license. Please contact our office to learn more.

Researchers

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