Immortalized clonal human myoblast line "M007"
Immortalized human myoblast cell line obtained from a healthy individual
Technology No. 2021-256
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Applications
- Research and testing of myogenesis
- Myotube development
- Differentiation
- Drug development
Technology Overview
Immortalized human myoblast cell line from primary myoblasts obtained from the muscle biopsy of a healthy individual.The cells were immortalized by overexpressing hTERT, CCND1 and a mutant CDK4, using two vectors pLV-hTERT-IRES-hygro and pbabe-cyclinD1+CDK4R24C from Addgene. These cells can grow indefinitely and can be differentiated into myotubes in vitro, by changing the medium to "differentiation medium", essentially withdrawing growth factors
Technical Information
Organism: Homo sapiens (human)
Tissue: Skeletal Muscle
Disease: Normal
Karyotype: 46XX [20/20 normal metaphase spreads]
Product format:Frozen
Desired Partnerships
This cell line is fully developed and available for license. Please contact our office to learn more.
Researchers
- Michael Kyba, PhD Professor, Department of Pediatrics
- Darko Bosnakovski, PhD Assistant Professor, Department of Pediatrics
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swap_vertical_circlelibrary_booksReferences (1)
- Darko Bosnakovski, Erik A Toso, Elizabeth T Ener, Micah D Gearhart, Lulu Yin, Felipe F Lüttmann, Alessandro Magli, Ke Shi, Johnny Kim, Hideki Aihara, Michael Kyba (October 2023), Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein, iScience, 26
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