Immortalized clonal human fibroblast line "F007"
Immortalized human fibroblast cell line obtained from a healthy individual
Technology No. 2021-257
Applications
- Wound healing and skin regeneration
- Tissue engineering
- Skin therapy
Technology Overview
Immortalized human fibroblast cell line from primary fibroblasts obtained from the muscle biopsy of a healthy individual.The cells were immortalized by overexpressing hTERT, CCND1 and a mutant CDK4, using two vectors pLV-hTERT-IRES-hygro and pbabe-cyclinD1+CDK4R24C from Addgene. These cells can grow indefinitely and can be differentiated into fat cells in vitro, by changing the medium to adipogenic differentiation medium.
Technical Information
Organism: Homo sapiens (human)
Tissue: Skeletal Muscle
Disease: Normal
Karyotype: 46XX [20/20 normal metaphase spreads]
Product format:Frozen
Desired Partnerships
This cell line is fully developed and available for license. Please contact our office to learn more
Researchers
- Michael Kyba, PhD Professor, Department of Pediatrics
- Darko Bosnakovski, PhD Assistant Professor, Department of Pediatrics