Fluorescence-based assay for probing lipid transfer activity
A sensitive, reproducible and quantitative assay to study lipid transfer using bicelles as model membranes.
- Lipid transfer assay kits for biological, medical, and bioengineering research
- Fluorescent-based assay kit
- Biophysical and membrane biochemistry research tool kit
- Drug discovery
Fluorescence based assay to identify and study lipid transfer proteins
Lipid composition of cell membranes plays an important role in determining the permeability, interaction with biomolecules, and stability of the membrane. Lipid composition is therefore tightly regulated by a number of factors, including lipid transfer proteins and interaction with lipid particles. Quantitative understanding of lipid transfer from one biomembrane to another has significant implications in discerning complex biophysical behavior of proteins and other biomolecules. Changes in the expression of certain lipid transfer proteins can also trigger biomedical-related events such as inflammation and programmed cell death processes (e.g. autophagy and necroptosis). Researchers at the University of Minnesota have created a new assay to quantify lipid transfer activity using a fluorescence resonance energy transfer (FRET)-based approach with bicelles. This technology can be used to:
determine if a protein of interest facilitates lipid transfer between membranes,
accurately determine the rate of lipid transfer, and
identify and evaluate drug candidates that can modulate lipid transfer activity.
This bicelle-based assay offers several advantages over liposome-based approach by providing higher stability and enabling high-throughput screening. Bicelles exhibit enhanced ‘shelf-life’ such as stability during refrigeration and avoidance of freeze-thaw changes that plague lipid vesicles. In addition, the small size of bicelles results in minimal light scattering artifacts increasing the reliability and reproducibility of FRET measurements. Lastly, this assay can be used to study lipid transfer between donor bicelles and a variety of acceptor model membranes (such as bicelles, liposomes, and micelles).
Potential Market Opportunity
The stability of this technology enables it to be the basis for a commercial benchtop kit that will allow researchers to investigate lipid transfer activity without needing to acquire expertise in lipid techniques.
Phase of DevelopmentProof of concept to study transfer activity of GLTP and CPTP with highly reproducible data. (GLTP - glycolipid transfer protein; CPTP - ceramide-1-phosphate transfer protein)
Features & Benefits
- FRET-based assay offers high sensitivity and accuracy
- FRET measurements offers safety advantages over traditional radioactive approaches by avoiding radioactive lipids needed to track the intermembrane transfer of lipids
- Assay can be used for identifying lipid transfer specificity
- Assay applicable for studying any protein/biomolecule of interest
- Applicable in high-throughput screening (e.g. plate reader)
This technology is now available for:
- Sponsored research
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