Cryopreservation method for millimeter scale biological samples using Cryomesh

A cryopreservation method that uses cryomesh and cryopreservation agents to preserve Drosophila embryos.
Technology No. 2021-019
IP Status: Provisional Patent Application Filed

Applications

  • Cryopreservation

Overview

Drosophila stocks must be maintained through the frequent and repeated transfer of breeding Drosophila. This manual maintenance is time consuming and costly; it places the stocks at risk for genetic drift or the accumulation of mutations; and it threatens the loss of a line from poor reproductive capacity, accidental mixing of stocks, or contaminations. To address this issue, researchers at the University of Minnesota have developed a simple and robust cryopreservation protocol for Drosophila melanogaster embryos such that the embryos can be stored in liquid nitrogen without requiring costly maintenance. The protocol uses cryoprotectant agents (CPA) and a novel cryomesh that facilitates handling of large quantities of samples.

Phase of Development

TRL: 3
Approach currently being tested in several drosophila strains. In vitro testing for islets.

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Researchers

  • swap_vertical_circlelibrary_booksReferences (1)
    1. Zhan, Li, Min-gang Li, Thomas Hays, and John Bischof. , Cryopreservation method for Drosophila melanogaster embryos, Nature communications 12, no. 1 (2021): 1-10.
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