Cryopreservation method for millimeter scale biological samples using Cryomesh
A cryopreservation method that uses cryomesh and cryopreservation agents to preserve Drosophila embryos.
Drosophila stocks must be maintained through the frequent and repeated transfer of breeding Drosophila. This manual maintenance is time consuming and costly; it places the stocks at risk for genetic drift or the accumulation of mutations; and it threatens the loss of a line from poor reproductive capacity, accidental mixing of stocks, or contaminations. To address this issue, researchers at the University of Minnesota have developed a simple and robust cryopreservation protocol for Drosophila melanogaster embryos such that the embryos can be stored in liquid nitrogen without requiring costly maintenance. The protocol uses cryoprotectant agents (CPA) and a novel cryomesh that facilitates handling of large quantities of samples.
Phase of DevelopmentTRL: 3
Approach currently being tested in several drosophila strains. In vitro testing for islets.
Desired PartnershipsThis technology is now available for:
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- Zhan, Li, Min-gang Li, Thomas Hays, and John Bischof. , Cryopreservation method for Drosophila melanogaster embryos, Nature communications 12, no. 1 (2021): 1-10.
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