Cryopreservation method for millimeter scale biological samples using Cryomesh
A cryopreservation method that uses cryomesh and cryopreservation agents to preserve Drosophila embryos.
Applications
- Cryopreservation
Overview
Drosophila stocks must be maintained through the frequent and repeated transfer of breeding Drosophila. This manual maintenance is time consuming and costly; it places the stocks at risk for genetic drift or the accumulation of mutations; and it threatens the loss of a line from poor reproductive capacity, accidental mixing of stocks, or contaminations. To address this issue, researchers at the University of Minnesota have developed a simple and robust cryopreservation protocol for Drosophila melanogaster embryos such that the embryos can be stored in liquid nitrogen without requiring costly maintenance. The protocol uses cryoprotectant agents (CPA) and a novel cryomesh that facilitates handling of large quantities of samples.
Phase of Development
TRL: 3Approach currently being tested in several drosophila strains. In vitro testing for islets.
Desired Partnerships
This technology is now available for:- License
- Sponsored research
- Co-development
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Researchers
- Thomas Hays, PhD, Professor, Department of Genetics
- John Bischof, PhD, Professor, Department of Mechanical Engineering
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swap_vertical_circlelibrary_booksReferences (1)
- Zhan, Li, Min-gang Li, Thomas Hays, and John Bischof. , Cryopreservation method for Drosophila melanogaster embryos, Nature communications 12, no. 1 (2021): 1-10.
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swap_vertical_circlecloud_downloadSupporting documents (1)Product brochureCryopreservation method for millimeter scale biological samples using Cryomesh.pdf