More Efficient PCR Thermal CyclingA thermal cycling device has been designed that can regulate the temperature of biological samples on a flat substrate, such as a microscope slide. Polymerase chain reaction (PCR) requires cycles of repeated heating and cooling of the reaction to amplify copies of a piece of DNA. Current PCR tools do not have slides that can be used in a thermal cycling device. This technology provides a thermal cycler that optimizes heat flow to and from an apparatus that is has a biological sample attached to a microscope slide or other flat surface.
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Microscope Slide Heating and Cooling
This technology is comprised of many parts that all work together to provide the necessary heating and cooling of the biological sample located on a microscope slide. The slide is fitted with a micro-fabricated heater and conductive pads are located on each side on the lower surface of the slide. Conductive pads located on the upper surface of the slide are connected to a resistive temperature sensor that helps to regulate the temperature of the substrate. The technology also has a fan located below where the slide is mounted to allow for rapid cooling to take place when necessary in the PCR reaction process.
FEATURES AND BENEFITS OF PCR THERMAL CYCLING DEVICE:
- Provides thermal cycling capability to a microscope slide
- Optimizes heat flow to and from apparatus
- All-in-one device allows for rapid heating and cooling of substrates
- Fitted with a temperature sensor for temperature regulation