Molecular Probe Identifies Kinase Drugs and Metabolic Activity
A recently designed molecular probe can
measure nucleosides. This high-throughput screening tool of kinase
drugs is a valuable diagnostic tool for measuring drug activity in
energy metabolism pathways. This small lanthanide-based probe binds
selectively and reversibly to adenosine triphosphate (ATP) and adenosine
diphosphate (ADP) with different affinities, which produces a weak
interaction that has an influence on the luminescence of the probe. The
most important benefit is that the probe is able to detect ATP at higher
concentrations, closer to the intracellular concentrations of 1-10mM
needed to screen inhibitors of low-affinity kinases.
Current Nucleoside Measuring Technologies
Nucleosides play crucial roles in energy metabolism within cells.
Current state of the art technologies for measuring nucleosides are often accompanied by high costs, inability to measure nucleosides at physiologically relevant concentrations, cumbersome reagents, and require radio-active labels.
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Affordable Molecular Probe
The molecular probe is affordable,
non-radioactive, label-free, antibody-free, generic, and enables the
study of large proteins without being dependent on cumbersome reagents.
The technology also enables time-resolved or time-gated luminescence
detection along with the ability to measure the accumulation of ADP
rather than the phosphorylation of a peptide.
ADVANTAGES OF THE MOLECULAR PROBE:
- Single reagent molecular probe - fluoresces differently depending upon which nucleoside it is binding with
- Can be used as a real-time fluorescent probe in assay systems
- Used for the kinetic study of enzymes and enzyme inhibition-able to screen all classes of kinases which utilize nucleosides
- Not dependent on cumbersome peptide phosphorylation reagents
- Enable study of large protein substrates
- Affordable, non-radioactive, antibody-free, and label-free